An article in J Food Protect. of September 2024, entitled ”Evaluation of methods for identifying poultry wing rinses with Salmonella concentrations greater than or equal to 10 CFU/mL,” deals with the need identified by the FSIS for simple, rapid methods that identify poultry wing rinse samples harboring Salmonella concentrations ≥ 10 CFU/mL. Samples were inoculated at levels from 0.22 to 3.79 log CFU/mL and then measured by 3-tube Most Probable Number (MPN), BioMerieux GENE-UP QUANT, Hygiena BAX SalQuant, and novel threshold methods. The MPN lower limit of quantification (LLQ) for Salmonella was –0.96 log CFU/mL. MPN overestimated the inoculated Salmonella level by 0.05 ± 0.35 log CFU/mL. The GENE-UP QUANT Salmonella method (LLQ = 1.00 log CFU/mL) underestimated the inoculated Salmonella level by 0.05 ± 0.51 log CFU/mL. The BAX SalQuant method (LLQ = 0.00 log CFU/mL) underestimated the inoculated Salmonella level by 1.21 ± 0.78 log CFU/mL. MPN was 93.1%, which is accurate for determining if Salmonella levels in poultry wing rinses were ≥ 10 CFU/mL. GENE-UP quantification was 86.1% accurate. BAX quantification had the lowest accuracy, 58.4%. PiLOT threshold test accuracies ranged from 83.2% for PiLOT-50 to 93.1% for PiLOT-86. The PiLOT threshold tests are simple and can be adapted to identify many environmental or food samples containing Salmonella exceeding any user defined concentration threshold.@ https://www.sciencedirect.com/science/article/pii/S0362028X24001467?dgcid=raven_sd_aip_email