The Journal of Food Protection published a manuscript (in press) entitled “Real-time detection of Foodborne Pathogens and Biofilm in the food processing environment with Bactiscan, a macro-scale fluorescence device.” Bactiscan is investigated as an alternative detection approach, a macro-scale and reagentless device that detects microbial contamination by activating the green fluorescence of glycoproteins in the bacterial cell wall. The detection capability of Bactiscan was tested on foodborne pathogens Escherichia coli, Listeria monocytogenes, Salmonella enterica, and Staphylococcus aureus. Detection by Bactiscan was possible to 1.20*106 colony forming units (CFU), compared to 1.36*104 CFU by ATP swab testing, where Bactiscan detection limits were defined by the concentration at which 50% of the samples were observed under the illumination of the device. Heat-killed and chlorine stressed E. coli and S. enterica caused a 2-log reduction in detection by ATP swab tests (p≤0.05), while detection by Bactiscan was unaffected (p≥0.05). In situ contamination studies determined that Bactiscan can detect microbial contamination on chicken, salmon, and yogurt samples with stronger fluorescence than a competitor’s UV torch. These data demonstrate that Bactiscan can effectively detect bacteria present in the food processing environment and can complement existing technologies to improve food industry cleaning practices and infection prevention. @ https://www.sciencedirect.com/science/article/pii/S0362028X25000638?dgcid=raven_sd_aip_email