Label-free droplet-based bacterial growth phenotype screening by a mini integrated microfluidic platform

An article was published in Sensors and Actuator B: Chemical Vol 385, 15 June 2023. Ge et al., Entitled “Label-free droplet-based bacterial growth phenotype screening by a mini integrated microfluidic platform.” Current measurements for strain growth phenotype are based on macroscopic culture and continuous turbidity monitoring followed by a growth curve with a long evaluation period. Rapid and accurate microbial screening, important in industrial breeding, enzyme directed evolution, and synthetic biology, is currently time-consulting. Fluorescence-activated droplet sorting (FADS) system offers a promising alternative for microbial screening with high-throughput analysis. For many methods, pre-labeling steps are required before use. A new, low-cost, label-free droplet-based mini integrated microfluidic platform was developed for bacterial growth phenotype screening based on the difference in droplet autofluorescence properties. Droplet provides a parallel culture unit for single cells, enabling high-throughput measurement of the growth rate of single-cell cultures in a short time. A low-cost and modularized microfluidic system is developed for bacterial growth phenotype screening in droplets, in which a flexible and on-demand droplet detector is designed to be compatible with microfluidic devices. @ https://www.sciencedirect.com/science/article/abs/pii/S0925400523004069?via%3Dihub

 

 

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