A new Method to detect low levels of viable Salmonella Enteritidis within 10 hours

A group of scientists from Portugal developed a new rapid method based on the amplification of the Salmonella bacteriophage vB_SenS_PVP-SE2, coupled with real-time PCR, for the detection of viable Salmonella Enteritidis in chicken samples. The method detected 0.22 fg/μL of pure virus DNA and a concentration of viral particles of 103 pfu/mL. The authors claim that after a short recovery step, the addition of bacteriophages to spiked chicken, a sensitivity of 8 cfu/25 g could be detected within 10 h, including the time for DNA extraction and qPCR analysis. Values above 92% were obtained for sensitivity, accuracy, positive and negative predictive value. The results obtained show that the new methodology might be a useful tool for the detection of Salmonella Enteritidis (one of the most common serovars associated with foodborne illnesses). @ https://www.sciencedirect.com/science/article/pii/S0956713518306388?d" rel="nofollow noopener noreferrer" target="_blank">https://www.sciencedirect.com/science/article/pii/S0956713518306388?d
Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples

Serovar Enteritidis represents 45.7% of all Salmonella reported human cases identified in Europe. Additionally, “minced meat and meat preparations fro…

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