A method using real-time multiplex PCR was developed to differentiate the four major Listeria monocytogenes serotypes in meat processing plants

Researchers at the University of Extremadura (Cáceres, Spain) have developed a rapid, specific, and sensitive method that has potential use in identifying sources of Listeria monocytogenes contamination in the meat industry. The scientists developed a quadruplex real-time quantitative PCR (qPCR) method able to differentiate the four predominant serotypes of L. monocytogenes (1/2a, 1/2b, 1/2c, and 4b) in isolates from meat processing plants and ready-to-eat (RTE) dry-cured meat products. The standard curves showed efficiency values ranging between 92.3% and 105.8% and R2 values >0.98. The specificity of the method was also confirmed by the comparison of the results with those obtained by a previously reported conventional multiplex PCR. Strains not ascribed to L. monocytogenes did not amplify any target genes linked to the four major serotypes studied. This method could be very useful for identifying sources of L. monocytogenes contamination in the meat industry or for epidemiological monitoring of persistent strains throughout the processing of RTE meat products. @ https://www.sciencedirect.com/science/article/pii/S0740002019309773?dgcid=rss_sd_all

 Development of a multiplex real-time PCR to differentiate the four major Listeria monocytogenes serotypes in isolates from meat processing plants
Development of a multiplex real-time PCR to differentiate the four major Listeria monocytogenes serotypes in isolates from meat processing plants

Listeria monocytogenes is an important foodborne pathogen, causative agent of listeriosis. The epidemiology and persistence of this bacterium in meat …

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