Validation of RapidCheck Select Salmonella Test Method for Detecting Low Levels of Salmonella species in Palm Oil

Objective

The RapidChek SELECT Salmonella test system (RC SS) is designed to detect Salmonella species in a variety of foods and environmental surfaces, and is now also validated to detect low levels of Salmonella spp. in palm oil. The test kit permits the presumptive detection and identification of Salmonella spp. in a minimum of 22 hours when the pathogen is present at low levels in food and environmental surfaces.

Challenges

Method Comparison Study (FDA BAM versus RapidChek) Salmonella Typhimurium ATCC 14028 spiked at 2.5 cfu/25g sample (n=20 per method) + 5 unincculated negative controls. Cultural confirmation of all samples regardless of presumptive results on XLD and HE agars (FDA BAM) FDA BAM Chapter 5: Salmonella    

Results

    

Conclusion

• RapidChek Select Salmonella detected Salmonella in palm oil with 100% sensitivity and specificity within 22 hours
• Proved equivalent to FDA BAM cultural method RapidChek will provide users with a rapid, reliable, cost effective tool for monitoring and controlling Salmonella species in palm oil

This poster was presented at the IAFP 2016 Annual meeting