Test for antibiotic resistance genes provides answers within an hour
An article was published in Chemistry World (https://www.chemistryworld.com/news/test-for-antibiotic-resistance-genes-provides-answers-within-an-hour/4011130.article)describing a test developed at the University of California, Irvine to diagnosing bacterial infection and whether the bacteria contain a variety of antibiotic resistance genes.
The test is sensitive (10–100 times better than existing methods) and provides the results within an hour. The assay can analyze whole blood and uses the polymerase chain reaction.
The test eliminates the need for sub-culturing of the blood. The method uses a microfluidic device that mixes the whole blood sample with PCR reagents, which work with whole blood, and then turn this mixture into millions of individual picolitre-sized droplets. After the PCR reaction, droplets containing particular genetic targets become fluorescent and are detected and quantified by a high throughput 3D particle counter. As the sample is being analyzed, the counter moves the sample up and down and rotates it. The test allows the use of one milliliter of blood and the higher volume increases the probability of detecting bacteria.
Very interesting development, soon, this method might be adapted to the food industry.