Gerold Schwarz Send the following additional Information:By: Dr. Gerold Schwarz, Produktmanager BIOTECON Diagnostics GmbH
Elimination of DNA form dead cells prior a PCR-Setup:Reagent D is designed for the rapid elimination of DNA from dead cells to avoid false-positive PCR results. The reagent contains a light sensitive substance which can penetrate the cell membranes of dead cells, whereas the outer membrane compartments of living cells can actively protect their cytosolic compartments.
- After a brief incubation of a freshly prepared enrichment culture with reagent D.
- The complete Assay is exposed 5 minutes to a high-power halogen light source.
- Isolate/ extract DNA
- Run PCR
- Final PCR Results and no or low false positive rates.