In the J. Food Protec, 2025, a publication available online on 13 February 2025 entitled “Biofilm forming abilities of Salmonella serovars isolated from clinically ill livestock at 48 and 168 hours, reported that Evaluation of cleaning and disinfection in preharvest environments has found little success in eradicating Salmonella biofilms. Disrupting the environmental survival of Salmonella via biofilm removal will be critical to reducing carriage in livestock reservoirs and the risk of foodborne illness. The objective of the study was to characterize the Biofilm forming abilities of Salmonellae relevant to livestock and human health. Eighty-one isolates from 8 serovars (S. Typhimurium, Heidelberg, Montevideo, Agona, Newport, Dublin, 4,[5],12:i:-, Enteritidis) were sourced from poultry and clinically ill cattle, swine, and equine. Strong biofilm formers comprised 84% of the isolates tested. Biofilm density was significantly greater at 168 hours versus 48 hours for all serovars except Dublin. Results suggest inconsistent cleaning likely allows for the establishment of biofilms in on-farm environments. Some serovars may pose a greater risk for rapid biofilm establishment. @ https://www.sciencedirect.com/science/article/pii/S0362028X25000183?dgcid=raven_sd_aip_email